From the mother colonies, 76 experimental territories of one hundred individuals were developed

From the mother colonies, 76 experimental territories of one hundred individuals were developed

2.step 1 Varieties studied and you can rearing criteria

Four territories regarding N. exitiosus away from similar models was amassed when you look at the Adelaide (Southern area Australia) into the later spring, amongst the 22nd off , towards a hill crest, where Eucalyptus woods was in fact the main plants. The mother colonies have been kept in the newest research for at least 2 weeks that have post libitum wood, insect nutritional elements (Vanderzant vitamin combination to own pests-Sigma) and you can salt mix W (MP biomedical) before the experiment to reduce eventual variability during the territories initial health condition.

Each experimental nest contains 70 large experts (phase 2–5, discover McMahan & Watson, 1975 ) and you can 30 slight soldiers. We utilized castes that will be sterile therefore we could analysis the effects of nutritional demands, independent regarding reproductive efforts. Termites have been located during the an excellent ten ? ten cm Petri bowl. One or two 3rd of your Petri pan is filled with 4% agar gel, to incorporate moisture and allow tunnelling. These husbandry has been utilized prior to now within species (Eutick, Veivers, O’Brien, & Slaytor, 1978 ). To prevent fungi infections, a solution out of fungicide (ten drops out-of Zaleton for every single litre) are dispersed into agar and allowed to deceased up until the termites were launched. For each and every fresh nest is actually moved to a different sort of nest all six weeks to stop attacks and you may dryness. …